The trim is just that. Cosmetic. I would not pull the windshield to do that unless you had some reason (you can see a leak?).
You will want to check the clips that hold the trim. If they are missing you would need to pull. I have yet to get one in without.
I do have the individual pieces or sell as a set.
https://914rubber.com/chrome-windshield-molding-set-1Just let me know if you want individual pieces.